Identification of natural inhibitors of human hepatocellular carcinomas using a systems biology approach
"School of Mechanical Engineering National Technical University of Athens"
Human hepatocellular carcinomas (HCC) develop in a milieu of inflammation. Since current therapeutic modalities are not effective, new prevention/treatment strategies are necessary. Plant compounds with anti-inflammatory properties may provide an alternative approach against HCC. This study seeks to identify such biomolecules and explore their action mechanisms by combining high throughput assays with a systemic analysis of HCC signalling networks. Cells will be exposed to test compounds in the presence or absence of inflammatory/growth stimuli and the levels of 30 secreted cytokines and 15 signaling phosphoproteins will be evaluated using custom multiplex assays on a Luminex 200 platform. Computational analysis will be subsequently applied to construct pathway maps linking cytokine secretion function to signalling activity. The biological significance of compound-mediated modifications will be evaluated in experimental models of HCC growth, metastasis and angiogenesis.
Our systemic approach would decipher the actions of effective compounds and pinpoint to novel therapeutic interventions for HCC.
This project was evaluated and funded as part of the Foundation’s effort to support research teams with all their members being under 40 years old.
Leonidas Alexopoulos, Assistant Professor, School of Mechanical Engineering, National Technical University of Athens
Mary Michailidou, Postdoctoral Researcher, Medical School, National and Kapodistrian University of Athens
Theodoros Sakellaropoulos, PhD Student, National and Kapodistrian University of Athens
Heleni Loutrari, Senior Research Scientist, Laboratory Teaching Staff, National and Kapodistrian University of Athens