Science

Programme "Scientific projects"

Development of an immunoassay for the quantification of a new marker for bacterial infections, the decapeptide proTα(100-109), in blood plasma

Department of Biology, School of Science, National and Kapodistrian University of Athens

2013

Prothymosin α (proTα) is a polypeptide essential for cell proliferation, while extracellularly it enhances cell-mediated immunity. Previous studies conducted by the team, showed that its immunoenhancing role is exerted by the carboxyterminal decapeptide proTα(100-109). Within 2 hours after apoptosis initiation, proTα is truncated by activated caspase-3, generating proTα(100-109). Unpublished data from the team showed that proTα(100-109) is excreted via an unknown mechanism and acts as a “danger signal” on immune cells. A sensitive and specific ELISA will be developed for the detection and quantification of proTα(100-109) in biological fluids. The presence of proTα(100-109) will be correlated in vitro with various types of cell-death in cell cultures. Using murine bacterial infection models, the in vivo production of proTα(100-109) will be studied. Finally, the levels of proTα(100-109) will be evaluated in patients’ plasma, to clinically assess an early diagnosis of bacterial infection and its differential diagnosis from non-infectious inflammatory disorders (e.g. autoinflammatory diseases).

Final Report (in Greek)

Coordinator:

Ourania Tsitsiloni, Assistant Professor in Immunology, Faculty of Biology, School of Science, National and Kapodistrian University of Athens (EKPA)

Project's Team:

Pinelopi Samara, PhD Candidate, Faculty of Biology, School of Science, National and Kapodistrian University of Athens (EKPA)

Eleni Tsitsami, Research Associate, First Pediatric Clinic, National and Kapodistrian University of Athens (EKPA)

Ioannis Voutsas, Biologist, Post-doctoral Researcher, Faculty of Biology, School of Science, National and Kapodistrian University of Athens (EKPA)

Anastasios Germenis, Professor in Immunology, Department of Medicine, School of Health Sciences, University of Thessaly

University Campus, Zografou

15701 Athens

Tel.: +30 210 72 74 397

Fax: +30 210 72 74 065

secr@biol.uoa.gr

www.biol.uoa.gr (in Greek)

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